R-spondin 3 蛋白, Human (125aa, HEK293, His)

RSPO3 is a potent activator of the canonical Wnt pathway and can bind to LGR4-6 receptors to initiate a complex with phosphorylated LRP6 and Frizzled receptors. This interaction activates the canonical Wnt pathway and upregulates target genes. R-spondin 3 Protein, Human (125aa, HEK293, His) is the recombinant human-derived R-spondin 3 protein, expressed by HEK293 , with C-His labeled tag. The total length of R-spondin 3 Protein, Human (125aa, HEK293, His) is 125 a.a., with molecular weight of 16 & (20-24) kDa, respectively.

R-Spondin 3 (RSPO3) 是经典 Wnt 信号通路的有效激活剂，作为 LGR4-6 受体的配体，在血管生成调节中发挥关键作用。与 LGR4-6（LGR4、LGR5 或 LGR6）结合后，所得复合物与磷酸化的 LRP6 和卷曲受体结合，并被细胞外 Wnt 受体激活。这种相互作用触发典型的 Wnt 信号通路，导致靶基因表达上调。RSPO3 还通过抑制 ZNRF3（Wnt 信号通路的重要组成部分）发挥多方面的调节作用，并充当卷曲 FZD8 和 LRP6 的配体。它还可能对 TGF-β 途径发挥负调节作用。在血管生成的背景下，RSPO3 成为关键参与者，通过激活内皮细胞中的非经典 Wnt 信号通路来控制血管稳定性和修剪。值得注意的是，RSPO3 表现出独立于 LGR4-6 受体放大 Wnt 信号通路的能力，可能是通过与 RNF43 和 ZNRF3 的直接拮抗相互作用。与 FZD8 和 LRP6 胞外结构域的相互作用，以及与 WNT1 和 LGR4、LGR5 和 LGR6 的结合，强调了 RSPO3 在 Wnt 信号传导调节中精心策划的复杂调节机制。

Measured by the ability to enhance BMP-2 induced alkaline phosphatase activity in MC3T3-E1 cells. The ED₅₀ for this effect is 1.787 μg/mL .

Human

HEK293

C-His

Q9BXY4-1 (Q22-V146)

84870  [NCBI]

QNASRGRRQRRMHPNVSQGCQGGCATCSDYNGCLSCKPRLFFALERIGMKQIGVCLSSCPSGYYGTRYPDINKCTKCKADCDTCFNKNFCTKCKSGFYLHLGKCLDNCPEGLEANNHTMECVSIV

16 & (20-24) kDa

Greater than 95% as determined by reducing SDS-PAGE

无菌粉末

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.